Hotspot

trnR-ACG-trnN-GUU

IRb · 109192-109500 · 309 bp

Back to Centrosema

Pi 0.0194

MarkerSeek score 63.90

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 309
variable_sites: 6
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	GGTACTCCGGGGAACAGATA	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.9	58.0	503-510 bp	1.0	80.3
trnR-ACG-trnN-GUU_p2	GGTACTCCGGGGAACAGATA	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.9	58.1	544-551 bp	1.0	79.5
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	450-457 bp	1.0	79.3
trnR-ACG-trnN-GUU_p4	GGTACTCCGGGGAACAGATA	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.9	57.8	467-474 bp	1.0	79.2
trnR-ACG-trnN-GUU_p5	GGTACTCCGGGGAACAGATA	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.9	58.2	454-461 bp	1.0	79.2