Hotspot

psbE-petL

LSC · 64564-65602 · 1039 bp

Back to Centrosema

Pi 0.016

MarkerSeek score 71.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1039
variable_sites: 15
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTACTCAGACCGA	50.0	45.5	57.8	57.9	1060-1155 bp	1.0	66.8
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTACTCAGACCGA	55.0	45.5	58.2	57.9	1055-1150 bp	1.0	66.4
psbE-petL_p3	CCTGCAATGAATAGGGAGGG	AGTCGTATCTTACTCAGACCGA	55.0	45.5	57.7	57.9	1134-1229 bp	1.0	65.7
psbE-petL_p4	TCTCCTGTGCTTCCAGACAT	AGTCGTATCTTACTCAGACCGA	50.0	45.5	58.4	57.9	1053-1148 bp	1.0	65.2
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	TGAGGTCATAGTTAAAACCGCT	50.0	40.9	57.8	57.5	1028-1123 bp	1.0	63.5