Hotspot

accD-psaI

LSC · 58943-59508 · 566 bp

Back to Centrosema

Pi 0.0195

MarkerSeek score 64.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 11
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGAGTTACTTCAGCTCCATGC	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.0	58.0	682-702 bp	1.0	73.0
accD-psaI_p2	TGAGTTACTTCAGCTCCATGC	AAGAAGCCATTGCAATTGCC	47.6	45.0	58.0	57.9	688-708 bp	1.0	72.4
accD-psaI_p3	GAGTTACTTCAGCTCCATGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.2	58.0	681-701 bp	1.0	67.0
accD-psaI_p4	GAGTTACTTCAGCTCCATGC	AAGAAGCCATTGCAATTGCC	50.0	45.0	56.2	57.9	687-707 bp	1.0	66.4
accD-psaI_p5	TGAGTTACTTCAGCTCCATGC	CCGGAAATACTAGGCCCACT	47.6	55.0	58.0	58.9	670-690 bp	1.0	66.3