Hotspot

trnQ-UUG-accD

LSC · 56981-57445 · 465 bp

Back to Centrosema

Pi 0.0272

MarkerSeek score 67.00

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: LSC
length_bp: 465
variable_sites: 11
pi: 0.0272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	TCCACACCTGTATTCTAACTTCC	55.0	43.5	58.1	57.6	501-549 bp	1.0	57.7
trnQ-UUG-accD_p2	GAGGTTCGAATCCTTCCGTC	CCACACCTGTATTCTAACTTCCT	55.0	43.5	58.1	57.6	500-548 bp	1.0	57.7
trnQ-UUG-accD_p3	CAAATCGAAAATGGGGCGTG	TCCACACCTGTATTCTAACTTCC	50.0	43.5	58.4	57.6	560-608 bp	1.0	55.2
trnQ-UUG-accD_p4	CAAATCGAAAATGGGGCGTG	CCACACCTGTATTCTAACTTCCT	50.0	43.5	58.4	57.6	559-607 bp	1.0	55.2
trnQ-UUG-accD_p5	TCAAATCGAAAATGGGGCGT	TCCACACCTGTATTCTAACTTCC	45.0	43.5	58.5	57.6	561-609 bp	1.0	54.5