Hotspot

psbK-trnQ-UUG

LSC · 56383-56908 · 526 bp

Back to Centrosema

Pi 0.0217

MarkerSeek score 63.50

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 526
variable_sites: 11
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	CAGAGTGACTACAAATACAGACCA	GACCAAAACCCGTTGCCTTA	41.7	50.0	58.0	58.7	607-608 bp	1.0	46.3
psbK-trnQ-UUG_p2	ACAGAGTGACTACAAATACAGACC	GACCAAAACCCGTTGCCTTA	41.7	50.0	58.0	58.7	608-609 bp	1.0	46.3
psbK-trnQ-UUG_p3	CAGAGTGACTACAAATACAGACCA	CGAATAACGGGACCAAAACC	41.7	50.0	58.0	57.1	617-618 bp	1.0	44.4
psbK-trnQ-UUG_p4	ACAGAGTGACTACAAATACAGACC	CGAATAACGGGACCAAAACC	41.7	50.0	58.0	57.1	618-619 bp	1.0	44.4
psbK-trnQ-UUG_p5	GGACAGAGTGACTACAAATACAGA	GACCAAAACCCGTTGCCTTA	41.7	50.0	57.7	58.7	610-611 bp	1.0	44.3