Hotspot

petN-trnC-GCA

LSC · 33950-34838 · 889 bp

Back to Centrosema

Pi 0.0105

MarkerSeek score 64.90

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 889
variable_sites: 9
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TGGTGGTGGGGTATTCTCAT	45.0	50.0	58.0	58.0	1029-1039 bp	1.0	83.0
petN-trnC-GCA_p2	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	942-952 bp	1.0	82.8
petN-trnC-GCA_p3	CAGCCCAAGCAAGACTTACT	TGGTGGTGGGGTATTCTCAT	50.0	50.0	57.8	58.0	1023-1033 bp	1.0	81.5
petN-trnC-GCA_p4	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.8	58.1	936-946 bp	1.0	81.3
petN-trnC-GCA_p5	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	960-970 bp	1.0	80.5