Hotspot

ycf1

IRb · 107778-115223 · 7446 bp

Back to Centropogon

Pi 0.0126

MarkerSeek score 52.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 7446
variable_sites: 89
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGTGAAAAAGTCCAGAACGC	ACCTCGCGGAGTAATACGTA	50.0	50.0	58.0	58.0	488-626 bp	1.0	89.7
ycf1_p2	CGTGAAAAAGTCCAGAACGC	GTTCGTCCAGGTCGTATACG	50.0	55.0	58.0	57.9	508-646 bp	1.0	89.2
ycf1_p3	AGGGCACTTCTTCAATTGGG	ACCTCGCGGAGTAATACGTA	50.0	50.0	58.1	58.0	704-782 bp	1.0	88.0
ycf1_p4	AAAGGGAAGAAAAGGCACGT	ACCTCGCGGAGTAATACGTA	45.0	50.0	57.9	58.0	790-868 bp	1.0	87.5
ycf1_p5	CGAGGATCCCGAAGAAAAGG	ACCTCGCGGAGTAATACGTA	55.0	50.0	58.1	58.0	960-1038 bp	1.0	86.9