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ORF308

LSC · 33080-33987 · 908 bp

Back to Centropogon

Pi 0.0199

MarkerSeek score 65.10

Primer available yes

Label ORF308

Feature Metadata

label_name: ORF308
feature_type: gene
region: LSC
length_bp: 908
variable_sites: 18
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF308_p1	CCGGTATTGAAACAAACCCG	TCATGGAGAGTCGATCCTGG	50.0	55.0	57.4	58.3	1062-1117 bp	1.0	77.9
ORF308_p2	CCGGTATTGAAACAAACCCG	AGGCTTAACACATGCACAGA	50.0	45.0	57.4	57.4	1021-1076 bp	1.0	75.9
ORF308_p3	CCGGTATTGAAACAAACCCG	CAGGCTTAACACATGCACAG	50.0	50.0	57.4	57.4	1022-1077 bp	1.0	75.4
ORF308_p4	CCGGTATTGAAACAAACCCG	GGCTTAACACATGCACAGAG	50.0	50.0	57.4	57.1	1020-1075 bp	1.0	73.1
ORF308_p5	TCTTCGTCAAAGTCGGAATCC	TCATGGAGAGTCGATCCTGG	47.6	55.0	58.0	58.3	1044 bp	0.5	49.2