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ORF222

LSC · 57339-58007 · 669 bp

Back to Centropogon

Pi 0.0224

MarkerSeek score 56.70

Primer available yes

Label ORF222

Feature Metadata

label_name: ORF222
feature_type: gene
region: LSC
length_bp: 669
variable_sites: 15
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF222_p1	TGATCGAACCGTCCAGTTTC	TCGTACGACCCCACTAAAGA	50.0	50.0	57.9	57.8	1420-1430 bp	1.0	79.3
ORF222_p2	AAAGCAGAAGCATAGACGCA	TCGTACGACCCCACTAAAGA	45.0	50.0	57.9	57.8	1521-1531 bp	1.0	79.0
ORF222_p3	TGCTAATGGGGGCTAAGACT	TCGTACGACCCCACTAAAGA	50.0	50.0	58.1	57.8	1614-1624 bp	1.0	78.9
ORF222_p4	TCGCAGTCTCACCTAGGATT	TCGTACGACCCCACTAAAGA	50.0	50.0	57.9	57.8	1267-1277 bp	1.0	78.9
ORF222_p5	CTAGAAAGTTTCTCCGGCCC	TCGTACGACCCCACTAAAGA	55.0	50.0	58.0	57.8	1636 bp	0.5	58.2