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ORF189

LSC · 11775-12344 · 570 bp

Back to Centropogon

Pi 0.0713

MarkerSeek score 76.10

Primer available yes

Label ORF189

Feature Metadata

label_name: ORF189
feature_type: gene
region: LSC
length_bp: 570
variable_sites: 40
pi: 0.0713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF189_p1	TGCTTTCTACCACAGCGTTT	GTCCCGGTAATCGAAGGTTC	45.0	55.0	58.0	58.1	993-1325 bp	1.0	86.3
ORF189_p2	TTGCTTTCTACCACAGCGTT	GTCCCGGTAATCGAAGGTTC	45.0	55.0	58.0	58.1	994-1326 bp	1.0	86.3
ORF189_p3	AGCGAACATCAATTGCAACG	GTCCCGGTAATCGAAGGTTC	45.0	55.0	58.0	58.1	1031-1363 bp	1.0	86.1
ORF189_p4	GCGAACATCAATTGCAACGA	GTCCCGGTAATCGAAGGTTC	45.0	55.0	58.0	58.1	1030-1362 bp	1.0	86.1
ORF189_p5	GATCCCTTTATCACGCTGCA	GTCCCGGTAATCGAAGGTTC	50.0	55.0	58.0	58.1	1498-1823 bp	1.0	85.0