Hotspot

rpl14-rpl16

LSC · 80302-80470 · 169 bp

Back to Centropogon

Pi 0.0265

MarkerSeek score 69.30

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 4
pi: 0.0265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCGACGATTGCTAGCTCCTA	ATGAAATCGGTGGCGTAACA	50.0	45.0	58.0	57.9	342-360 bp	1.0	83.8
rpl14-rpl16_p2	TCGACGATTGCTAGCTCCTA	TCGGTGGCGTAACAGAAAAT	50.0	45.0	58.0	57.8	336-354 bp	1.0	83.3
rpl14-rpl16_p3	TCGACGATTGCTAGCTCCTA	AAATCGGTGGCGTAACAGAA	50.0	45.0	58.0	57.8	339-357 bp	1.0	83.2
rpl14-rpl16_p4	TCGACGATTGCTAGCTCCTA	AATCGGTGGCGTAACAGAAA	50.0	45.0	58.0	57.8	338-356 bp	1.0	83.2
rpl14-rpl16_p5	TCGACGATTGCTAGCTCCTA	ATCGGTGGCGTAACAGAAAA	50.0	45.0	58.0	57.8	337-355 bp	1.0	83.2