Hotspot

rps14-psaB

LSC · 43504-43626 · 123 bp

Back to Centropogon

Pi 0.0325

MarkerSeek score 71.20

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 4
pi: 0.0325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	GCCGGAAGCTTGCTTATTTC	ACTTATGCGGCGTTCTTGAT	50.0	45.0	57.8	58.0	278 bp	1.0	79.4
rps14-psaB_p2	GCCGGAAGCTTGCTTATTTC	TTGATTGCCTCGACATCAGG	50.0	50.0	57.8	58.0	263 bp	1.0	79.4
rps14-psaB_p3	GCCGGAAGCTTGCTTATTTC	CCCTTTCCATTGTCCAAGCA	50.0	50.0	57.8	58.4	340 bp	1.0	76.4
rps14-psaB_p4	GCCGGAAGCTTGCTTATTTC	CGTTCTTGATTGCCTCGACA	50.0	50.0	57.8	58.6	268 bp	1.0	75.1
rps14-psaB_p5	GCCGGAAGCTTGCTTATTTC	TACTTATGCGGCGTTCTTGA	50.0	45.0	57.8	57.3	279 bp	1.0	74.3