Hotspot

rpoC2-rpoC1

LSC · 26301-26465 · 165 bp

Back to Centropogon

Pi 0.0187

MarkerSeek score 66.10

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 3
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	TGTGAAAGACCAGATTGGCC	GCGAAATCGAAGAGGCCATA	50.0	50.0	58.1	58.1	247-252 bp	1.0	80.8
rpoC2-rpoC1_p2	TGTGAAAGACCAGATTGGCC	GAGGCCATACAGGGGTTTTC	50.0	55.0	58.1	58.2	236-241 bp	1.0	80.1
rpoC2-rpoC1_p3	TGTGAAAGACCAGATTGGCC	TATCGCGAAATCGAAGAGGC	50.0	50.0	58.1	58.2	251-256 bp	1.0	79.9
rpoC2-rpoC1_p4	TGTGAAAGACCAGATTGGCC	TTTCCCAGGCCTGTTCCTAT	50.0	50.0	58.1	58.3	220-225 bp	1.0	79.6
rpoC2-rpoC1_p5	TGTGAAAGACCAGATTGGCC	TTTTCCCAGGCCTGTTCCTA	50.0	50.0	58.1	58.5	221-226 bp	1.0	77.8