Hotspot

atpF-atpH

LSC · 18744-19168 · 425 bp

Back to Centropogon

Pi 0.0141

MarkerSeek score 63.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 6
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GGTTGTCGCATTAGCCCTTT	50.0	50.0	57.9	58.8	537-538 bp	1.0	73.3
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	ATTTATGGCCTGGTTGTCGC	50.0	50.0	57.9	58.9	548-549 bp	1.0	72.8
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GTTGTCGCATTAGCCCTTTT	50.0	45.0	57.9	57.0	536-537 bp	1.0	71.9
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCGGAGGGGAAAATACGAG	50.0	57.9	57.9	58.0	608-609 bp	1.0	71.7
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GGTTGTCGCATTAGCCCTT	50.0	52.6	57.9	58.1	537-538 bp	1.0	70.9