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trnQ (UUG)-psbK

LSC · 12603-13115 · 513 bp

Back to Centropogon

Pi 0.034

MarkerSeek score 70.70

Primer available yes

Label trnQ (UUG)-psbK

Feature Metadata

label_name: trnQ (UUG)-psbK
feature_type: igs
region: LSC
length_bp: 513
variable_sites: 16
pi: 0.034
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ (UUG)-psbK_p1	GAACCTTCGATTACCGGGAC	CGGGCAATTTGGCAAAGAAA	55.0	45.0	58.1	58.1	650-753 bp	1.0	84.3
trnQ (UUG)-psbK_p2	TCGATTACCGGGACCAAAAC	CGGGCAATTTGGCAAAGAAA	50.0	45.0	57.9	58.1	644-747 bp	1.0	84.1
trnQ (UUG)-psbK_p3	CTTCGATTACCGGGACCAAA	CGGGCAATTTGGCAAAGAAA	50.0	45.0	57.6	58.1	646-749 bp	1.0	81.7
trnQ (UUG)-psbK_p4	GATTACCGGGACCAAAACCC	CGGGCAATTTGGCAAAGAAA	55.0	45.0	58.5	58.1	642-745 bp	1.0	80.6
trnQ (UUG)-psbK_p5	TTCGATTACCGGGACCAAAA	CGGGCAATTTGGCAAAGAAA	45.0	45.0	57.1	58.1	645-748 bp	1.0	78.0