Hotspot

ORF189-trnQ (UUG)

LSC · 12345-12530 · 186 bp

Back to Centropogon

Pi 0.272

MarkerSeek score 74.20

Primer available yes

Label ORF189-trnQ (UUG)

Feature Metadata

label_name: ORF189-trnQ (UUG)
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 50
pi: 0.272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF189-trnQ (UUG)_p1	CTCGGGGCTTATCGTTTTCA	CGGGTCAAATTCCTAGCACA	50.0	50.0	58.0	57.9	1110-1538 bp	1.0	88.6
ORF189-trnQ (UUG)_p2	TTGTGAGTGAGCCACCTTTC	GTCCCGGTAATCGAAGGTTC	50.0	55.0	58.0	58.1	618-949 bp	1.0	88.1
ORF189-trnQ (UUG)_p3	GAATGCCCCATGCCAAAATC	GTCCCGGTAATCGAAGGTTC	50.0	55.0	58.0	58.1	760-1085 bp	1.0	87.9
ORF189-trnQ (UUG)_p4	CTCGGGGCTTATCGTTTTCA	GTCCCGGTAATCGAAGGTTC	50.0	55.0	58.0	58.1	787-1112 bp	1.0	87.8
ORF189-trnQ (UUG)_p5	CTCGGGGCTTATCGTTTTCA	GTTTTGGTCCCGGTAATCGA	50.0	50.0	58.0	57.9	793-1118 bp	1.0	87.5