Hotspot

atpF

LSC · 51065-52381 · 1317 bp

Back to Centrolobium

Pi 0.00076

MarkerSeek score 51.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1317
variable_sites: 1
pi: 0.00076
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGTCTATCCATAAGAGGAGATCA	TGCACGAATGGTTACCATGA	39.1	45.0	55.2	57.5	1434-1435 bp	1.0	40.1
atpF_p2	AGTCTATCCATAAGAGGAGATCA	TCTCATCTGCACGAATGGTT	39.1	45.0	55.2	57.2	1441-1442 bp	1.0	40.1
atpF_p3	AGTCTATCCATAAGAGGAGATCA	ATCTGCACGAATGGTTACCA	39.1	45.0	55.2	57.2	1437-1438 bp	1.0	40.1
atpF_p4	AGTCTATCCATAAGAGGAGATCA	CTGCACGAATGGTTACCATG	39.1	50.0	55.2	57.2	1435-1436 bp	1.0	40.1
atpF_p5	AGTCTATCCATAAGAGGAGATCAT	TGCACGAATGGTTACCATGA	37.5	45.0	55.7	57.5	1434-1435 bp	1.0	40.1