Hotspot

trnL-UAG-ccsA

SSC · 118318-118437 · 120 bp

Back to Centrolobium

Pi 0.0345
MarkerSeek score 75.40
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
120
variable_sites
4
pi
0.0345
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA ACAATTGATACGACCGAAAACG 50.0 40.9 57.9 57.7 239-243 bp 1.0 63.1
trnL-UAG-ccsA_p2 AAATTGGTAGACACGCTGCT ACAATTGATACGACCGAAAACG 45.0 40.9 57.8 57.7 246-250 bp 1.0 62.6
trnL-UAG-ccsA_p3 GGTAGACACGCTGCTCTTAG ACAATTGATACGACCGAAAACG 55.0 40.9 57.8 57.7 241-245 bp 1.0 62.5
trnL-UAG-ccsA_p4 GTAGACACGCTGCTCTTAGG ACAATTGATACGACCGAAAACG 55.0 40.9 57.8 57.7 240-244 bp 1.0 62.5
trnL-UAG-ccsA_p5 TTTCGAGTAAGCCGCTATGG ACAATTGATACGACCGAAAACG 50.0 40.9 57.8 57.7 268-272 bp 1.0 62.1