Hotspot

rpl32-trnL-UAG

SSC · 117405-118237 · 833 bp

Back to Centrolobium

Pi 0.0072

MarkerSeek score 54.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 833
variable_sites: 6
pi: 0.0072
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAGGGGATATTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	955-978 bp	1.0	77.9
rpl32-trnL-UAG_p2	AGAGGGGATATTGGACAGCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.8	57.8	948-971 bp	1.0	77.4
rpl32-trnL-UAG_p3	AGAGGGGATATTGGACAGCA	ATAAGATGCCATGCCGCTAC	50.0	50.0	57.8	58.2	1007-1030 bp	1.0	77.4
rpl32-trnL-UAG_p4	AGAGGGGATATTGGACAGCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.8	57.8	954-977 bp	1.0	77.4
rpl32-trnL-UAG_p5	AGAGGGGATATTGGACAGCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.8	57.8	953-976 bp	1.0	77.4