Hotspot

trnP-UGG-psaJ

LSC · 69605-70240 · 636 bp

Back to Centrolobium

Pi 0.00789

MarkerSeek score 63.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 5
pi: 0.00789
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	738-739 bp	1.0	78.4
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	737-738 bp	1.0	78.4
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	749-750 bp	1.0	78.3
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	748-749 bp	1.0	78.3
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	751-752 bp	1.0	78.3