Hotspot

trnS-UGA-psbC

LSC · 26571-26812 · 242 bp

Back to Centrolobium

Pi 0.0455

MarkerSeek score 78.60

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 242
variable_sites: 11
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.0	58.4	441-442 bp	1.0	78.4
trnS-UGA-psbC_p2	GCTGAGTGGTTGATAGCTCC	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	433-434 bp	1.0	78.2
trnS-UGA-psbC_p3	GGCTGAGTGGTTGATAGCTC	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	434-435 bp	1.0	78.2
trnS-UGA-psbC_p4	GGTTGATAGCTCCGGTCTTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	426-427 bp	1.0	78.2
trnS-UGA-psbC_p5	GGCTTTTTGGAGAGATGGCT	TAGGTCATTTATGGCACGCG	50.0	50.0	58.2	58.4	450-451 bp	1.0	77.3