Hotspot

ycf3-psaA

LSC · 19090-19961 · 872 bp

Back to Centrolobium

Pi 0.00354

MarkerSeek score 63.60

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 3
pi: 0.00354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.0	57.9	1001-1020 bp	1.0	81.3
ycf3-psaA_p2	AGCCCTTACCCCTGGTAATT	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.0	57.9	1000-1019 bp	1.0	81.3
ycf3-psaA_p3	AGCTATAGCCCTTACCCCTG	TGCCCATTCCTCGAAAGAAG	55.0	50.0	58.0	57.9	1007-1026 bp	1.0	81.0
ycf3-psaA_p4	AGCTATAGCCCTTACCCCTG	GCCCATTCCTCGAAAGAAGT	55.0	50.0	58.0	57.9	1006-1025 bp	1.0	81.0
ycf3-psaA_p5	TTGGTTGAAGATCACAGGGC	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.1	57.9	965-984 bp	1.0	80.7