Hotspot

psbA-trnK-UUU

LSC · 1444-1767 · 324 bp

Back to Centrolobium

Pi 0.00658

MarkerSeek score 61.20

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 2
pi: 0.00658
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AACACCAAACCATCCGATGT	CGGCTTGGGGAGAGATTTTT	45.0	50.0	57.7	58.2	518-538 bp	1.0	79.8
psbA-trnK-UUU_p2	ACGGTTTTCAGTGCTGGTTA	CGGCTTGGGGAGAGATTTTT	45.0	50.0	57.7	58.2	494-514 bp	1.0	79.5
psbA-trnK-UUU_p3	GCTCTCGCGTCTCTCTAAAA	CGGCTTGGGGAGAGATTTTT	50.0	50.0	57.5	58.2	443-463 bp	1.0	78.2
psbA-trnK-UUU_p4	TACAGAAGCGACCCCATAGG	CGGCTTGGGGAGAGATTTTT	55.0	50.0	58.6	58.2	468-488 bp	1.0	77.7
psbA-trnK-UUU_p5	CCATCCGATGTAAAGACGGT	CGGCTTGGGGAGAGATTTTT	50.0	50.0	57.4	58.2	509-529 bp	1.0	77.3