Hotspot

ycf1

SSC · 122791-127968 · 5178 bp

Back to Centaurea

Pi 0.0069

MarkerSeek score 36.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5178
variable_sites: 114
pi: 0.0069
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCTAAATAGTAGACACAGGTAA	TGGTTGGATAATTGGTCACA	36.4	40.0	52.1	54.5	195 bp	1.0	44.5
ycf1_p2	CCTAAATAGTAGACACAGGTAAA	TGGTTGGATAATTGGTCACA	34.8	40.0	53.0	54.5	195 bp	1.0	44.5
ycf1_p3	CCTAAATAGTAGACACAGGTAA	TTGGTTGGATAATTGGTCACA	36.4	38.1	52.1	55.4	196 bp	1.0	44.5
ycf1_p4	CCTAAATAGTAGACACAGGTAAA	TTGGTTGGATAATTGGTCACA	34.8	38.1	53.0	55.4	196 bp	1.0	44.5
ycf1_p5	CCTAAATAGTAGACACAGGTAA	TTTGGTTGGATAATTGGTCACA	36.4	36.4	52.1	56.2	197 bp	1.0	44.5