Hotspot

ndhA

SSC · 118663-120814 · 2152 bp

Back to Centaurea

Pi 0.00486

MarkerSeek score 50.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2152
variable_sites: 35
pi: 0.00486
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCATAGGGAGCATATCGTGA	CCCCGGGGTTTATTAATTTGC	47.6	47.6	58.1	57.5	2355-2361 bp	1.0	60.8
ndhA_p2	ACTCAGTTACCATAGGGAGCA	CCCCGGGGTTTATTAATTTGC	47.6	47.6	57.9	57.5	2363-2369 bp	1.0	60.8
ndhA_p3	ACCATAGGGAGCATATCGTG	CCCCGGGGTTTATTAATTTGC	50.0	47.6	56.6	57.5	2355-2361 bp	1.0	58.3
ndhA_p4	CCATAGGGAGCATATCGTGA	CCCCGGGGTTTATTAATTTGC	50.0	47.6	56.3	57.5	2354-2360 bp	1.0	56.0
ndhA_p5	ACCATAGGGAGCATATCGTGA	CCCCCGGGGTTTATTAATTTG	47.6	47.6	58.1	56.9	2356-2362 bp	1.0	55.7