Hotspot

rpl20-rps12

LSC · 68428-69172 · 745 bp

Back to Centaurea

Pi 0.00225

MarkerSeek score 48.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 7
pi: 0.00225
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCTCCGAGCGATATATCCCC	CAGCGTCGAGGAACATGTAC	55.0	55.0	57.9	58.7	807-811 bp	1.0	74.9
rpl20-rps12_p2	TCTCCGAGCGATATATCCCC	AGCGTCGAGGAACATGTACT	55.0	50.0	57.9	58.8	806-810 bp	1.0	74.2
rpl20-rps12_p3	TCTCCGAGCGATATATCCCC	TCAGCGTCGAGGAACATGTA	55.0	50.0	57.9	58.8	808-812 bp	1.0	74.1
rpl20-rps12_p4	TCTCCGAGCGATATATCCCC	GCGTCGAGGAACATGTACTA	55.0	50.0	57.9	57.2	805-809 bp	1.0	74.0
rpl20-rps12_p5	TTCTACGTCTCCGAGCGATA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.8	58.7	814-818 bp	1.0	73.5