Hotspot

ndhC-trnV-UAC

LSC · 49720-50871 · 1152 bp

Back to Centaurea

Pi 0.00616
MarkerSeek score 59.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1152
variable_sites
21
pi
0.00616
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.7 58.1 1106-1326 bp 1.0 82.0
ndhC-trnV-UAC_p2 CGGTCCTTTGCTAATCGGAG CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.4 58.1 1108-1328 bp 1.0 81.1
ndhC-trnV-UAC_p3 CTCCGGTCCTTTGCTAATCG CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.4 58.1 1111-1331 bp 1.0 81.1
ndhC-trnV-UAC_p4 GGTCCTTTGCTAATCGGAGC CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.7 58.1 1107-1327 bp 1.0 78.9
ndhC-trnV-UAC_p5 GTCCTTTGCTAATCGGAGCT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 1091-1311 bp 1.0 77.4