Hotspot

atpH-atpF

LSC · 26173-26539 · 367 bp

Back to Centaurea

Pi 0.00619

MarkerSeek score 51.90

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 9
pi: 0.00619
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GCCTGGTTGTAGCATTAGCA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.2	57.9	440-479 bp	1.0	81.4
atpH-atpF_p2	GCCTGGTTGTAGCATTAGCA	GTGGCCCAAAGAAACGAAAG	50.0	50.0	58.2	57.9	441-480 bp	1.0	80.8
atpH-atpF_p3	GCCTGGTTGTAGCATTAGCA	ATTAAACCCGAAACTCCCGG	50.0	50.0	58.2	57.9	471-510 bp	1.0	80.7
atpH-atpF_p4	TGGCCTGGTTGTAGCATTAG	TGGCCCAAAGAAACGAAAGA	50.0	45.0	57.6	57.9	442-481 bp	1.0	80.1
atpH-atpF_p5	GCCTGGTTGTAGCATTAGCA	AGTGGCCCAAAGAAACGAAA	50.0	45.0	58.2	58.2	442-481 bp	1.0	80.0