Hotspot

trnE-UUC-rpoB

LSC · 11672-12595 · 924 bp

Back to Centaurea

Pi 0.00697

MarkerSeek score 48.50

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 25
pi: 0.00697
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GAGAGAAGTCGTAACCGCTG	TCTGATTAAATCCAGGTATTGTGG	55.0	37.5	58.1	56.5	939-985 bp	1.0	46.1
trnE-UUC-rpoB_p2	GAGAGAAGTCGTAACCGCTG	TCAATGAACCTACAAAATCCTTCA	55.0	33.3	58.1	56.5	970-1016 bp	0.909091	42.2
trnE-UUC-rpoB_p3	AACCCTCGTTATCTCCGTGA	TCAATGAACCTACAAAATCCTTCA	50.0	33.3	58.2	56.5	992-1038 bp	0.909091	42.2
trnE-UUC-rpoB_p4	ACCCTCGTTATCTCCGTGAA	TCAATGAACCTACAAAATCCTTCA	50.0	33.3	58.2	56.5	991-1037 bp	0.909091	42.2
trnE-UUC-rpoB_p5	TGAACCCTCGTTATCTCCGT	TCAATGAACCTACAAAATCCTTCA	50.0	33.3	58.2	56.5	994-1040 bp	0.909091	42.2