Hotspot

ycf1

IRb · 114351-115103 · 753 bp

Back to Cenostigma

Pi 0.00403

MarkerSeek score 62.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 753
variable_sites: 3
pi: 0.00403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCAATAGAAAAGAAAAAGGGGG	GGGTGGTCGAATTTCTTC	39.1	50.0	57.5	53.0	894-903 bp	1.0	41.1
ycf1_p2	TTGCAATAGAAAAGAAAAAGGGGG	GGGTGGTCGAATTTCTTC	37.5	50.0	58.1	53.0	895-904 bp	1.0	41.1
ycf1_p3	GCAATAGAAAAGAAAAAGGGGG	GGGTGGTCGAATTTCTTC	40.9	50.0	55.8	53.0	893-902 bp	1.0	41.1
ycf1_p4	TGCAATAGAAAAGAAAAAGGGG	GGGTGGTCGAATTTCTTC	36.4	50.0	55.1	53.0	894-903 bp	1.0	41.1
ycf1_p5	TTGCAATAGAAAAGAAAAAGGGG	GGGTGGTCGAATTTCTTC	34.8	50.0	55.9	53.0	895-904 bp	1.0	41.1