Hotspot

ndhA

SSC · 124430-126770 · 2341 bp

Back to Cenostigma

Pi 0.00558

MarkerSeek score 58.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2341
variable_sites: 13
pi: 0.00558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	42.9	50.0	56.3	57.6	2554-2562 bp	1.0	55.2
ndhA_p2	AACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	40.9	50.0	57.0	57.6	2555-2563 bp	1.0	53.2
ndhA_p3	TGAACCCAGTTACCATAAGGA	GTCCCCCGGGTTTTATCAAT	42.9	50.0	56.0	57.6	2557-2565 bp	1.0	52.9
ndhA_p4	GAACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	43.5	50.0	58.1	57.6	2556-2564 bp	1.0	52.5
ndhA_p5	TGAACCCAGTTACCATAAGGAAC	GTCCCCCGGGTTTTATCAAT	43.5	50.0	58.1	57.6	2557-2565 bp	1.0	52.5