Hotspot

rps12-rps7

IRa · 145639-145691 · 53 bp

Back to Cenostigma

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRa
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	CTGTGGAAAGCCGTATTCGA	ATTGGATCGGATTTTGCGGT	50.0	45.0	58.0	58.2	208 bp	1.0	78.4
rps12-rps7_p2	TCTGTGGAAAGCCGTATTCG	ATTGGATCGGATTTTGCGGT	50.0	45.0	58.0	58.2	209 bp	1.0	78.4
rps12-rps7_p3	CTGTGGAAAGCCGTATTCGA	TGGATCGGATTTTGCGGTTT	50.0	45.0	58.0	58.5	206 bp	1.0	76.7
rps12-rps7_p4	CTGTGGAAAGCCGTATTCGA	TTGGATCGGATTTTGCGGTT	50.0	45.0	58.0	58.5	207 bp	1.0	76.7
rps12-rps7_p5	TCTGTGGAAAGCCGTATTCG	TGGATCGGATTTTGCGGTTT	50.0	45.0	58.0	58.5	207 bp	1.0	76.7