Hotspot

rps3-rps19

LSC · 88166-88814 · 649 bp

Back to Cenostigma

Pi 0.0109

MarkerSeek score 65.40

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 7
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	TTAAACTTCCGGGGACATGC	50.0	50.0	57.5	58.2	737-784 bp	1.0	76.3
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	TTAAACTTCCGGGGACATGC	50.0	50.0	58.6	58.2	731-778 bp	1.0	75.4
rps3-rps19_p3	TGGTTGTGCAAACCAAAGAG	TTAAACTTCCGGGGACATGC	45.0	50.0	57.0	58.2	775-822 bp	1.0	72.2
rps3-rps19_p4	TCTGAAACCGAGCGGATTTA	TTAAACTTCCGGGGACATGC	45.0	50.0	57.0	58.2	730-777 bp	1.0	71.6
rps3-rps19_p5	GTCTGAAACCGAGCGGATT	TTAAACTTCCGGGGACATGC	52.6	50.0	57.9	58.2	731-778 bp	1.0	70.8