Hotspot

petD-rpoA

LSC · 82023-82227 · 205 bp

Back to Cenostigma

Pi 0.039

MarkerSeek score 74.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 8
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTTGGGTATTGGAGCAACA	TGGAACATTTTCGCATAGAAGA	50.0	36.4	58.1	56.1	335 bp	1.0	50.0
petD-rpoA_p2	TGGTTGGGTATTGGAGCAAC	TGGAACATTTTCGCATAGAAGA	50.0	36.4	58.1	56.1	336 bp	1.0	50.0
petD-rpoA_p3	CCCATTTCGTCGTCCAGTAG	TGGAACATTTTCGCATAGAAGA	55.0	36.4	58.1	56.1	394 bp	1.0	49.7
petD-rpoA_p4	CAGTAGCGACAACCGTCTTT	TGGAACATTTTCGCATAGAAGA	50.0	36.4	58.2	56.1	380 bp	1.0	48.6
petD-rpoA_p5	CCCATTTCGTCGTCCAGTAG	TCGCATAGAAGATGTAAAACGT	55.0	36.4	58.1	55.9	384 bp	1.0	48.6