Hotspot

psaJ-rpl33

LSC · 71328-71821 · 494 bp

Back to Cenostigma

Pi 0.0101

MarkerSeek score 62.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 5
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TACTCTATGGTTCGGGGCTT	CACTGTTTCGAACACAACTGG	50.0	47.6	57.8	57.9	652-674 bp	1.0	71.0
psaJ-rpl33_p2	GTGGCACCAGTGGTAAGTAC	CACTGTTTCGAACACAACTGG	55.0	47.6	58.2	57.9	669-691 bp	1.0	70.7
psaJ-rpl33_p3	TGGCACCAGTGGTAAGTACT	CACTGTTTCGAACACAACTGG	50.0	47.6	58.3	57.9	668-690 bp	1.0	70.1
psaJ-rpl33_p4	TACTCTATGGTTCGGGGCTT	CGGATATCTTTACCCTTGGCC	50.0	52.4	57.8	58.2	608-630 bp	1.0	69.7
psaJ-rpl33_p5	GTGGCACCAGTGGTAAGTAC	CGGATATCTTTACCCTTGGCC	55.0	52.4	58.2	58.2	625-647 bp	1.0	69.4