Hotspot

petA-psbJ

LSC · 66625-67831 · 1207 bp

Back to Cenostigma

Pi 0.00581

MarkerSeek score 62.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1207
variable_sites: 7
pi: 0.00581
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1344-1360 bp	1.0	75.7
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1349-1365 bp	1.0	75.2
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.4	57.6	1353-1369 bp	1.0	74.8
petA-psbJ_p4	ACGTGTCCAAGGTCTCTTGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.9	57.6	1345-1361 bp	1.0	70.6
petA-psbJ_p5	ATCCGTTACGTGTCCAAGGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	59.0	57.6	1352-1368 bp	1.0	69.4