Hotspot

ndhC-trnV-UAC

LSC · 54847-55469 · 623 bp

Back to Cenostigma

Pi 0.0202

MarkerSeek score 60.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 12
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGAGAAGCGGCCATAAACTG	TGCACGTTGGGTCTGTAAAA	50.0	45.0	58.0	58.0	1215-1241 bp	1.0	82.1
ndhC-trnV-UAC_p2	TTGTTACTGTGCCTGCTGTT	TGCACGTTGGGTCTGTAAAA	45.0	45.0	57.9	58.0	1347-1373 bp	1.0	81.7
ndhC-trnV-UAC_p3	CGTGGTACCAGTCCATAACG	TGCACGTTGGGTCTGTAAAA	55.0	45.0	58.1	58.0	1297-1323 bp	1.0	81.5
ndhC-trnV-UAC_p4	TGGTTCAGGCATTTGCTCAT	TGCACGTTGGGTCTGTAAAA	45.0	45.0	58.1	58.0	1397-1423 bp	1.0	81.4
ndhC-trnV-UAC_p5	AGAGAAGCGGCCATAAACTG	GCACAAGGGCTATAGCTCAG	50.0	55.0	58.0	58.1	1800-1826 bp	1.0	80.6