Hotspot

psbZ-trnG-GCC

LSC · 39605-40126 · 522 bp

Back to Cenostigma

Pi 0.014

MarkerSeek score 69.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 522
variable_sites: 7
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.0	57.7	677-731 bp	1.0	80.5
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	680-734 bp	1.0	78.9
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	673-727 bp	1.0	78.8
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.3	57.7	678-732 bp	1.0	78.6
psbZ-trnG-GCC_p5	TTCTCCTGATGGTTGGTCGA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	58.4	57.7	676-730 bp	1.0	77.7