Hotspot

trnR-UCU-atpA

LSC · 10925-11267 · 343 bp

Back to Cenostigma

Pi 0.0488

MarkerSeek score 70.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 16
pi: 0.0488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.0	58.0	1112-1190 bp	1.0	81.9
trnR-UCU-atpA_p2	CCTAGCCTTCCAAGCTAACG	GCGAGTTGCTCAAACAATCC	55.0	50.0	58.1	58.0	1115-1193 bp	1.0	81.7
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	CGAAGCTTATCCGGGAGATG	50.0	55.0	58.0	57.9	1513-1591 bp	1.0	81.1
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	ATCCCTCCAAACAAGCACAG	50.0	50.0	58.0	58.1	1577-1655 bp	1.0	80.9
trnR-UCU-atpA_p5	AGCCTTCCAAGCTAACGATG	GATCCCTCCAAACAAGCACA	50.0	50.0	58.0	58.1	1578-1656 bp	1.0	80.9