Hotspot

ndhG-ndhI

SSC · 111971-112156 · 186 bp

Back to Cenchrus

Pi 0.0327

MarkerSeek score 61.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 186
variable_sites: 14
pi: 0.0327
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCAGCGAAAAGGCAGAATA	TGGAATTCAAGAACGATTACTGA	50.0	34.8	58.2	56.0	286-347 bp	1.0	48.0
ndhG-ndhI_p2	CCCAGCGAAAAGGCAGAATA	TTGGAATTCAAGAACGATTACTGA	50.0	33.3	58.2	56.6	287-348 bp	1.0	48.0
ndhG-ndhI_p3	CCCAGCGAAAAGGCAGAATA	TGGAATTCAAGAACGATTACTG	50.0	36.4	58.2	54.5	286-347 bp	1.0	48.0
ndhG-ndhI_p4	CCCAGCGAAAAGGCAGAATA	TGGAATTCAAGAACGATTACTGAT	50.0	33.3	58.2	56.4	286-347 bp	1.0	48.0
ndhG-ndhI_p5	CCCAGCGAAAAGGCAGAATA	TTGGAATTCAAGAACGATTACTG	50.0	34.8	58.2	55.3	287-348 bp	1.0	48.0