Hotspot

rpl32-trnL-TAG

SSC · 106766-107259 · 494 bp

Back to Cenchrus

Pi 0.0222

MarkerSeek score 51.10

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 494
variable_sites: 28
pi: 0.0222
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	AGGAGCTCCCTTGTTTCGTA	GATCCGCACAAAAGAGTTGC	50.0	50.0	58.4	58.0	1654-1825 bp	1.0	84.9
rpl32-trnL-TAG_p2	AGGAGCTCCCTTGTTTCGTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	1075-1246 bp	1.0	84.7
rpl32-trnL-TAG_p3	AGGAGCTCCCTTGTTTCGTA	ATGTCCCGAAGAAACCCAAC	50.0	50.0	58.4	58.1	1388-1559 bp	1.0	84.6
rpl32-trnL-TAG_p4	AGGAGCTCCCTTGTTTCGTA	CCGAAGAAACCCAACGAGAA	50.0	50.0	58.4	58.1	1383-1554 bp	1.0	84.3
rpl32-trnL-TAG_p5	AGGAGCTCCCTTGTTTCGTA	AATGATTAGAAGAGCGGCGG	50.0	50.0	58.4	58.1	1682-1853 bp	0.916667	80.4