Hotspot

rpl23-psaI

LSC · 57400-57781 · 382 bp

Back to Cenchrus

Pi 0.0207

MarkerSeek score 61.10

Primer available yes

Label rpl23-psaI

Feature Metadata

label_name: rpl23-psaI
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 31
pi: 0.0207
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-psaI_p1	AGCATTGGGTCGAACTCTTC	CCAAGATAACTGGAAGCCCC	50.0	55.0	57.9	57.9	1093-1177 bp	1.0	86.6
rpl23-psaI_p2	GCATTGGGTCGAACTCTTCT	CCAAGATAACTGGAAGCCCC	50.0	55.0	57.9	57.9	1092-1176 bp	1.0	86.6
rpl23-psaI_p3	TGTACAAGCCCGTAACGAAG	CCAAGATAACTGGAAGCCCC	50.0	55.0	57.9	57.9	1574-1665 bp	1.0	86.5
rpl23-psaI_p4	TGTACAAGCCCGTAACGAAG	GCTAATAAATAGGCCCGCGA	50.0	50.0	57.9	57.9	1671-1762 bp	1.0	86.3
rpl23-psaI_p5	TGTACAAGCCCGTAACGAAG	AGCTAATAAATAGGCCCGCG	50.0	50.0	57.9	57.9	1672-1763 bp	1.0	86.3