Hotspot

trnF-GAA-ndhJ

LSC · 48610-49199 · 590 bp

Back to Cenchrus

Pi 0.0196

MarkerSeek score 67.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 38
pi: 0.0196
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	ATGCACAAGAAAGGGTCAGG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	722-777 bp	1.0	86.5
trnF-GAA-ndhJ_p2	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	712-767 bp	1.0	86.0
trnF-GAA-ndhJ_p3	GCACAAGAAAGGGTCAGGAT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	720-775 bp	1.0	85.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	669-724 bp	0.916667	83.2
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	659-714 bp	0.916667	82.7