Hotspot

trnC-GCA-rpoB

LSC · 18991-20216 · 1226 bp

Back to Cenchrus

Pi 0.0178

MarkerSeek score 64.10

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1226
variable_sites: 74
pi: 0.0178
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	TTATTGATTAGGCGGCACCC	CCGGGTATTGTGGACATTCC	50.0	55.0	58.0	58.3	1276-1347 bp	1.0	85.8
trnC-GCA-rpoB_p2	TTATTGATTAGGCGGCACCC	CGGGTATTGTGGACATTCCC	50.0	55.0	58.0	58.3	1275-1346 bp	1.0	85.8
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1239-1310 bp	1.0	85.5
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1238-1309 bp	1.0	85.5
trnC-GCA-rpoB_p5	TTATTGATTAGGCGGCACCC	TCAAGTTCTTCTGCCAAGCC	50.0	50.0	58.0	58.4	1342-1413 bp	1.0	85.0