Hotspot

trnY-GTA-trnD-GTC

LSC · 15675-16029 · 355 bp

Back to Cenchrus

Pi 0.0129

MarkerSeek score 62.60

Primer available yes

Label trnY-GTA-trnD-GTC

Feature Metadata

label_name: trnY-GTA-trnD-GTC
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 20
pi: 0.0129
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GTA-trnD-GTC_p1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	58.0	58.5	471-479 bp	1.0	81.1
trnY-GTA-trnD-GTC_p2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	438-446 bp	1.0	79.0
trnY-GTA-trnD-GTC_p3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	437-445 bp	1.0	78.8
trnY-GTA-trnD-GTC_p4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	50.0	50.0	58.0	57.0	470-478 bp	1.0	76.9
trnY-GTA-trnD-GTC_p5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.2	58.5	439-447 bp	1.0	75.6