Hotspot

rps16-trnQ-TTG

LSC · 5583-6705 · 1123 bp

Back to Cenchrus

Pi 0.0517

MarkerSeek score 73.00

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 107
pi: 0.0517
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	AAGTCGCACGTTGCTTTCTA	CGAAAACTTACAGCGGCTTG	45.0	50.0	58.1	58.0	1203-1784 bp	1.0	88.9
rps16-trnQ-TTG_p2	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGTTACTC	45.0	55.0	58.1	58.5	644-1217 bp	1.0	87.1
rps16-trnQ-TTG_p3	TCATGTCCTTCAAGTCGCAC	GGGTTTTGGTCCCGTTACTC	50.0	55.0	58.2	58.5	655-1228 bp	1.0	86.6
rps16-trnQ-TTG_p4	AAGTCGCACGTTGCTTTCTA	TGACAGGCATAACATCCACG	45.0	50.0	58.1	58.0	1387-1729 bp	0.916667	85.8
rps16-trnQ-TTG_p5	TCATGTCCTTCAAGTCGCAC	TGACAGGCATAACATCCACG	50.0	50.0	58.2	58.0	1398-1740 bp	0.916667	85.2