Hotspot

ycf3

LSC · 44076-46135 · 2060 bp

Back to Celtis

Pi 0.00296
MarkerSeek score 53.90
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
2060
variable_sites
20
pi
0.00296
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 GAAAAAGAATTCTAAACTGAAACT TCAAATTCCTTTGTTTGTGGGA 25.0 36.4 52.2 56.6 2202-2217 bp 1.0 41.6
ycf3_p2 GAAAAAGAATTCTAAACTGAAACT ATCAAATTCCTTTGTTTGTGGGA 25.0 34.8 52.2 57.1 2203-2218 bp 1.0 41.6
ycf3_p3 GAAAAAGAATTCTAAACTGAAACT TCAAATTCCTTTGTTTGTGGGAT 25.0 34.8 52.2 57.1 2202-2217 bp 1.0 41.6
ycf3_p4 AGAAAAAGAATTCTAAACTGAAAC TCAAATTCCTTTGTTTGTGGGA 25.0 36.4 52.2 56.6 2203-2218 bp 0.9 37.4
ycf3_p5 AGAAAAAGAATTCTAAACTGAAAC TCAAATTCCTTTGTTTGTGGGAT 25.0 34.8 52.2 57.1 2203-2218 bp 0.9 37.4