Hotspot

clpP

LSC · 72359-74528 · 2170 bp

Pi 0.00278

MarkerSeek score 53.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2170
variable_sites: 25
pi: 0.00278
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GCATATTTGCGGAAATCCCTC	ACGCTTTCACATCAAAGTGA	47.6	40.0	57.7	55.9	2252-2264 bp	0.9	49.9
clpP_p2	GCATATTTGCGGAAATCCCT	ACGCTTTCACATCAAAGTGA	45.0	40.0	56.5	55.9	2252-2264 bp	0.9	48.4
clpP_p3	GCATATTTGCGGAAATCCCTC	ACCAATTTACGCTTTCACATCA	47.6	36.4	57.7	56.9	2260-2272 bp	0.9	41.8
clpP_p4	GCATATTTGCGGAAATCCCTC	TACGCTTTCACATCAAAGTGA	47.6	38.1	57.7	55.8	2253-2265 bp	0.9	41.5
clpP_p5	GCATATTTGCGGAAATCCCT	ACCAATTTACGCTTTCACATCA	45.0	36.4	56.5	56.9	2260-2272 bp	0.9	40.4