Hotspot

accD-psaI

LSC · 60418-61398 · 981 bp

Back to Celtis

Pi 0.00521
MarkerSeek score 55.70
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
981
variable_sites
15
pi
0.00521
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GATCCAATCGTCCCACGTAA CCATTGCAATTGCCGGAAAT 50.0 45.0 57.4 58.0 1150-1186 bp 1.0 76.2
accD-psaI_p2 TCCAATCGTCCCACGTAATC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.4 58.0 1148-1184 bp 1.0 76.2
accD-psaI_p3 CCAATCGTCCCACGTAATCT CCATTGCAATTGCCGGAAAT 50.0 45.0 57.4 58.0 1147-1183 bp 1.0 76.2
accD-psaI_p4 GATCCAATCGTCCCACGTAA AGAAACCATTGCAATTGCCG 50.0 45.0 57.4 57.9 1155-1191 bp 1.0 75.8
accD-psaI_p5 TCCAATCGTCCCACGTAATC AGAAACCATTGCAATTGCCG 50.0 45.0 57.4 57.9 1153-1189 bp 1.0 75.8