Hotspot

rps4-trnT-UGU

LSC · 47763-48321 · 559 bp

Back to Celtis

Pi 0.00874
MarkerSeek score 60.90
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
559
variable_sites
15
pi
0.00874
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 CCCCCAGACGACGTATTTTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.9 57.9 671-732 bp 1.0 82.3
rps4-trnT-UGU_p2 CCCCCAGACGACGTATTTTT GATGGTCATCGGTTCGACTC 50.0 55.0 57.9 58.2 640-701 bp 1.0 82.2
rps4-trnT-UGU_p3 GGTTTCGAAGATCGCTTCCA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.3 57.9 725-786 bp 1.0 80.6
rps4-trnT-UGU_p4 TGGTTTCGAAGATCGCTTCC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.3 57.9 726-787 bp 1.0 80.6
rps4-trnT-UGU_p5 GGTTTCGAAGATCGCTTCCA GATGGTCATCGGTTCGACTC 50.0 55.0 58.3 58.2 694-755 bp 1.0 80.5